The effect of priming and aging on the activity of antioxidant enzymes and the mobility of seed reserves in French bean (Phaseolus vulgaris L.)

To examine the effect of priming and aging on the activity of antioxidant enzymes, mobility and use efficiency of seed reserves in French bean, a factorial experiment was performed in a completely randomized design with three replications. Treatments included aging (control without aging, 88% and 78% of germination of control) and priming (control, hydro-priming, priming by gibberellin and salicylic acid). The results showed that aging reduced germination percentage. Priming reduced the effect of aging and improved germination percentage. By increasing aging, seed reserves use efficiency (SRUE), seed reserves remobilization efficiency (SRRE) and fraction of used seed reserves (FUSR) were reduced, but respiration index (SR) and residual seed dry weight (RSDW) were increased. SRUE reduction was about 30% compared to the control treatment of aging and seed SR in gibberellin pre-treatment was higher about 24% compared to the control treatment without priming. The total seed protein content in gibberellin pre-treatment and without aging was increased about 32 percent. The peroxidase activity in gibberellin treatment and 88% aging level compared to the control showed an increase about 57%. The most superoxide dismutase activity (SOD, 175.2 unit mg-1 protein) was observed in priming with salicylic acid and 88% aging level. The maximum amount of malondialdehyde (MDA, 1.46 mmol g-1 FW) was related to the treatment with no priming and 78% aging level. In general, using gibberellin pre-treatment strengthened weak bean seeds physiologically and the treatment can be used to increase weak seed vigor.

کلیدواژه ها:

Salicylic acid ، antioxidant ، priming ، gibberellin ، seed reserves ، aging ، French bean