Synthesis and cytotoxicity investigation of synthetic nano oxali-palladium by turmeric extract using green chemistry

Introduction: Plant extracts involve secondary metabolites such as phenolic acid, flavonoid, Alkaloid and terpenoids. These compounds have responsibility to reduce ions into bulky nanoparticles. Different parts of plants such as stem, root, seed, fruit, leaf and flower are used to synthesis of metal nanoparticles in different forms and sizes by biological achievements. Turmeric is a gold herb, which is achieved from rhizome of curcuma longa. Different compounds are separated from this plant such as polyphenols, terpenes, diterpenes, sterol and alkaloids.Aim: The aim of this study is design, synthesis, characterization and anticancer activity of biocompatible oxali-Palladium nanoparticles using Curcuma longa extract against human colorectal cancer cell line of HCT116.Material and methods: At first, the alcoholic extract of Curcuma longa was prepared and then, the extract incubated with oxali-palladium solution in incubator shaker at500 C for 24 h. Physical and chemical properties such as diameter and surface charge of synthetic nanoparticles were studied using dynamic light scattering (DLS) Also electronic microscopy (SEM) is used to evaluate the morphology and diameter of nanoparticles. Finally, the anticancer activity and cytotoxicity of nanoparticles was investigated using MTT assay against HCT116 cell line. Results: Samples of synthetic nanoparticles which are produced by green chemistry method have shown significant changing in their color after 24h incubation time. The color changing shows that palladioum ions reduction by turmeric extracts in optimum synthetic condition. Also, the results of DLS studies suggest the formation of nanoparticles with diameter of 100 nm and 0.198 polydispersity. To confirm the morphology and the size of synthetic nanoparticles witch are synthesized by turmeric extracts, SEM study is done and the results showed the homogeneous spherical particles which are about 93 nm in their sizes. At the end, the cytotoxicity studies of synthetic nanoparticles against HCT116 Colorectal cancer cell line using MTT assay represents time and doze-depended response in colorectal cancer mortality induction.Conclusion: According to the results, it can be concluded that synthetic nano oxali-palladium by turmeric extracts are homogeneous and spherical in nano sizes and due to the use of any chemical agentsin synthetic steps, it can be a suitable candidate for colorectal cancer treatment.

کلیدواژه ها:

Oxali-Palladium Nanoparticles ، Turmeric Extract ، Green Chemistry ، Colorectal Cancer