Effect of Chitosan-Polycaprolactone nanofibrous scaffolds on burn wound healing in canine model

Introduction: Natural-synthetic polyblend nanofibers have attracted numerous research interests especially in regenerative medicine due to their high biological, physical, mechanical properties. On the other hand, natural-synthetic nanofibrous scaffolds would mimic natural ECM both in structure similarity and in biochemical properties [1]. Materials and methods: In this study, poly (caprolactone) (Mw 80 KDa), chitosan (Cs) (Mw 1000 KDa) and poly (vinyl alcohol) (PVA) (Mw 72 KDa) were used. PCL: Cs: PVA blend solution were prepared from 5% chitosan in 80% acetic acid, 10% PVA in distilled water and 10% PCL in 90% acetic acid. The solution was electrospun under condition of: 15kV applied voltage, 15cm distance, and 1ml/hr extrusion rate [2,3]. SEM micrographs were used for morphological investigations. In vivo study were carried out on five mixed-breed male dogs. Four 2 cm × 2cm full-thickness burn wounds were generated on the dorsal trunk. Half of the burn wounds were designated as the test by applying nanofibrous scaffolds and the rest as the control wounds. The healing process was evaluated on days 7, 14, 21, 30 and 90 post-operating. The macroscopic and microscopic investigations were carried out to measure the wounds areas and to track healing rate respectively. Result and conclusion: The presence of PCL in blend with chitosan leads to high biological properties along with maintaining physical integrity and avoiding chemical cross-linking. Macroscopic results showed good aspect healing effect of scaffolds in compared with control wounds especially after 21 days post operating. Pathological studies showed that the healing rates of the wounds covered with the scaffolds were much rapid compare to untreated wounds in control group. As the general result, the scaffold would help the healing process about 50% faster at final time point. Overall, PCL: Cs: PVA nanofibrous web could be considered as promising materials for wounds healings.

کلیدواژه ها:

Nanofibrous scaffold ، Chitosan ، burn ، healing