The association study of APOE Ɛ2 Ɛ3 Ɛ4, IL-6-572GC, IL6-174GC, ACE InsertionDeletion, and eNOS4b4a Polymorphisms in Iranian Stroke patients

Introduction: Based on the first population-based study of stroke in a Middle East region of Iran (Mashhad),the incidence of stroke in Iran is considerably greater than in most Western countries. Among the most widelyinvestigated genes involved inflammation, lipid metabolism, nitric oxide release, and reninangiotensinaldosteronesystems. In this Study we will analysis the genetic background for these gens in Persianstroke Population.Methods: Stroke subtypes were diagnosed by computed tomography scans and magnetic resonance imaging in200 Stroke Patient. Additionally, 222 control subjects were matched in some stroke risk factors. In our geneassociation study, was selected genes related with dependent systems in stroke, such as,ACE I/D ,IL6 -572 &-174 G> C,APOE e2/e3/e4 and eNOS 4b/a in, renin-angiotensin-aldosterone systems, inflammation, lipidmetabolism, and nitric oxide release. In these variations detected with semi nested PCR, RFLP-PCR-genotypediscrimination & Real Time PCR, multiplex tetra primers ARMS PCR and PCR techniques.Results: The ACE I/I and I allele, ApoEe2/e3, genotypes distribution in stroke patients differed significantlyfrom controls (P=0.031,P=0.026,P=0.015, respectively). Also, there are strong association between hemorrhagicsubtype and e2/e3, ACE I/I polymorphisms (p=0.001,P=0.024). Finally, ACE I/I and I allele associated betweenall subtype of stroke in the rage rang of 35 till 55 (age range of incidence).Conclusion: We report that there is a significant correlation between the ACE I/I, APOEe2/e3 and East Persianpopulation and Stroke. There are the high possibilities that I/I and e2/e3 Genotypes play a role in thedevelopment of the hemorrhagic Stroke.

کلیدواژه ها:

Stroke ، PCR-RFLP ، ARMS-PCR ، genetic variants