PH-Sensitive Chitosan based Nanohydrogel for Selective Photodynamic Therapy of Cancer
محل انتشار: اولین کنگره ملی نانو فناوری در علوم سلامت
سال انتشار: 1397
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 486
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شناسه ملی سند علمی:
NHSMED01_009
تاریخ نمایه سازی: 18 آذر 1398
چکیده مقاله:
Background and Objective :Photodynamic therapy (PDT) has attracted considerable attention in cancer therapy due to its negligible drug resistance, non-invasive nature, and excellent therapeutic effects.1 Photosensitizers (PSs) play a crucial role in PDT, by producing cytotoxic reactive oxygen species (ROS).2 Porphyrins and their derivatives are widely used as PSs for PDT treatment of cancer3Materials and Method :The chitosan was dissolved in 3 mL water containing 1% v/v acetic acid. As a typical hydrogel preparation, a solution of cross-linker was prepared by dissolving 0.002 g NH2-TPP (2% w/w) and 0.002 g TRIPOD (2% w/w) in 3mL DMF. For various hydrogels, the amount of NH2-TPP varied from 0.006 g to 0.002 g. The prepared NH2-TPP and TRIPOD solutions were added to chitosan solution at the same time and the mixture was shaken, and gelation happened within ∼30 minutes at 70 °C. 007 g of FA dissolved in 2 ml of dimethylsulfoxide (DMSO) was activated by 0.003 g of NHS and 0.007 g of DCC at room temperature for 12 h until FA was well dissolved.Finding :Herein, as a continuation of our research into the design, preparation and PDT investigation of hydrogels, we developed a facile approach to prepare a biocompatible self-healing, cell-specific and pH-sensitive hydrogel using chitosan conjugated with a porphyrin PS bridged with triazine groups (CS/NH2-TPP/FA) via a Schiff base reaction and folate (FA) functionality on the surface of the hydrogels in order to obtain a cancer cell targeting feature for photodynamic applications. The FA receptor is generally overexpressed in most cancer cells.Cytotoxicity and phototoxicity of CS/NH2-TPP and CS/NH2-TPP/FA hydrogels using human breast cancer MCF-7 cells as modal and immortalized human liver cancer HepG2 cells as a control show that the newly designed hydrogels were demonstrated to possess an excellent capability to selectively kill cancer cells with minimal side effectsConclusion :a chitosan-based hydrogel conjugated with tetrakis(4-aminophenyl)porphyrin (NH2-TPP) and 2,4,6-tris(p-formylphenoxy)-1,3,5-triazine via Schiff base linkage, functionalized with folate was designed and synthesized as a pH-sensitive, self-healable and injectable targeted PS delivery system.. Spectroscopic characterizations revealed that aggregation of porphyrin in aqueous media was eliminated due to diminished π stacking interaction of porphyrin in 3D cross-linked hydrogel structure. Hydrogel 3D microporous structure efficiently transfers the excitation energy to the porphyrin unit, yielding improvement singlet oxygen releases.
کلیدواژه ها:
نویسندگان
simin belali
Department of Chemistry, Faculty of Science, Arak University, Arak ۳۸۱۵۶-۸-۸۳۴۹, Iran
alireza karimi
Department of Chemistry, Faculty of Science, Arak University, Arak ۳۸۱۵۶-۸-۸۳۴۹, Iran Department of Nanosciences & Nanotechnology, Arak University, Arak, Iran.
mahnaz hadizadeh
Department of Biotechnology, Iranian Research Organization for Science and Technology Department of Chemistry, Faculty of Science, Arak University