PH-Sensitive Chitosan based Nanohydrogel for Selective Photodynamic Therapy of Cancer

سال انتشار: 1397
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 486

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شناسه ملی سند علمی:

NHSMED01_009

تاریخ نمایه سازی: 18 آذر 1398

چکیده مقاله:

Background and Objective :Photodynamic therapy (PDT) has attracted considerable attention in cancer therapy due to its negligible drug resistance, non-invasive nature, and excellent therapeutic effects.1 Photosensitizers (PSs) play a crucial role in PDT, by producing cytotoxic reactive oxygen species (ROS).2 Porphyrins and their derivatives are widely used as PSs for PDT treatment of cancer3Materials and Method :The chitosan was dissolved in 3 mL water containing 1% v/v acetic acid. As a typical hydrogel preparation, a solution of cross-linker was prepared by dissolving 0.002 g NH2-TPP (2% w/w) and 0.002 g TRIPOD (2% w/w) in 3mL DMF. For various hydrogels, the amount of NH2-TPP varied from 0.006 g to 0.002 g. The prepared NH2-TPP and TRIPOD solutions were added to chitosan solution at the same time and the mixture was shaken, and gelation happened within ∼30 minutes at 70 °C. 007 g of FA dissolved in 2 ml of dimethylsulfoxide (DMSO) was activated by 0.003 g of NHS and 0.007 g of DCC at room temperature for 12 h until FA was well dissolved.Finding :Herein, as a continuation of our research into the design, preparation and PDT investigation of hydrogels, we developed a facile approach to prepare a biocompatible self-healing, cell-specific and pH-sensitive hydrogel using chitosan conjugated with a porphyrin PS bridged with triazine groups (CS/NH2-TPP/FA) via a Schiff base reaction and folate (FA) functionality on the surface of the hydrogels in order to obtain a cancer cell targeting feature for photodynamic applications. The FA receptor is generally overexpressed in most cancer cells.Cytotoxicity and phototoxicity of CS/NH2-TPP and CS/NH2-TPP/FA hydrogels using human breast cancer MCF-7 cells as modal and immortalized human liver cancer HepG2 cells as a control show that the newly designed hydrogels were demonstrated to possess an excellent capability to selectively kill cancer cells with minimal side effectsConclusion :a chitosan-based hydrogel conjugated with tetrakis(4-aminophenyl)porphyrin (NH2-TPP) and 2,4,6-tris(p-formylphenoxy)-1,3,5-triazine via Schiff base linkage, functionalized with folate was designed and synthesized as a pH-sensitive, self-healable and injectable targeted PS delivery system.. Spectroscopic characterizations revealed that aggregation of porphyrin in aqueous media was eliminated due to diminished π stacking interaction of porphyrin in 3D cross-linked hydrogel structure. Hydrogel 3D microporous structure efficiently transfers the excitation energy to the porphyrin unit, yielding improvement singlet oxygen releases.

نویسندگان

simin belali

Department of Chemistry, Faculty of Science, Arak University, Arak ۳۸۱۵۶-۸-۸۳۴۹, Iran

alireza karimi

Department of Chemistry, Faculty of Science, Arak University, Arak ۳۸۱۵۶-۸-۸۳۴۹, Iran Department of Nanosciences & Nanotechnology, Arak University, Arak, Iran.

mahnaz hadizadeh

Department of Biotechnology, Iranian Research Organization for Science and Technology Department of Chemistry, Faculty of Science, Arak University