Effects of Cannabinoid Receptor Modulation on Voltage SAG in Cerebellar Purkinje Cells Under Whole Cell Patch Clamp Recording

Cannabinoid receptors are part of the endocannabinoid system which are involved in different physiological procedures including memory, painsensation and appetite. In the Whole-cell patch-clamp recording, Voltage SAG is a short period decrease in RMS voltage which can be caused by quick change of loads like motor startup, or short circuit. This property can be affected by changes in channels modulated byspecific receptors or ligands so can be one of comparison factors in pharmacological researchs. The purpose of this study was to investigate the effects of cannabinoid receptor modulators on voltage SAG in cerebellar purkinje cells under whole patch clamp recording.Materials and Methods: The voltage SAG in current clap mode here after IP injection of Harmaline, agonist WIN55, 212 2 and antagonists AM251 and SR171614a (Rimonobant) then slice of cerebellar vermis prepared and under direct vision of purkinje cells and confirmingthe patch, recording done and in offline mode the sag ratios were caculated by Digidata 1440 and pclamp 10.1. The data were analysed by prism v6. Results: Voltage SAG in response to – 0.5 nA stimulation charge in to the patch electrode showed significant increase in WINgroup comparison to control and Harmaline groups. This voltage in response to– 0.4 nA stimulation indicatedsignificant increase in WIN and Rimonobant group. Also, this result gained at – 0.3 nA stimulation. In -0.2 nA stimulation, voltage SAG had significant increase in WIN and AM251 groups. Voltage SAG in -0.1 nA had significant increase in all the groups. Conclusion: According to the results, the changes in the agonist group are higher than in others but antagonists showed improvement of that toward control group can be benefit effects on harmaline toxicity alongside of our previous behavioral study.