PuraMatrix Hydrogel Improved Proliferation of Motor Neuron-Like Cells

Development of neural tissue engineering approach for differentiation of stem cells into spinal motor neurons in the injury site isa new perspective for neurodegenerative diseases. Numerous scaffolds produced from a variety of biomaterials can be used to improve cell differentiation in the field of tissue engineering. Materials and Methods: We obtained neural stem cells-derived motor neuron-like cells (MNLCs) from rat adipose tissue and evaluate proliferation and differentiation on 2D and 3D (PuraMatrix hydrogel) culture systems. Results:Our results indicated that 3D environment increase in the expression of mature motor neuron markers. Also results of the current study showed that proliferation and adhesion of MNLCs in 3D environment is more than 2D. Conclusion: Taken together, this studysuggested that 3D environment can be considered as a potent therapeutic option for neural tissue regeneration.