Effect of Intraperitoneal Injection of Conditioned Medium of Mesenchymal Stem Cells in the Chronic Constriction Injury (CCI) Model of Neuropathic Pain in Male Rat

Neuropathic pain is one of the most important chronic pain results from injury to nervous system. Inflammatory mediators are involved in creating neuropathic pain. Mesenchymal stem cells (MSCs) by having anti-inflammatory properties can be a potential option in alleviating or treatment of neuropathic pain. Hence, this study aimed to investigate the effect of MSCs supernatant on neuropathic pain in chronic constriction injury (CCI) model. Materials and Methods: In this study, 24 male Wistar rats (6-8 weeks old, 200-250 gr) was used and randomly divide in 3 experimental group (Sham, CCI+MSCs and CCI+DMEM). MSCs were isolated from rats’ tibia bone marrow and culturedin laboratory. The MSCs supernatant was harvested at passage 5. Neuropathic pain was induced by CCI method. Experimental group received intraperitoneally 1 ml of MSCs supernatant on days -1, 1 and 2. Control group received DMEM as the same manner. In all groups, mechanical allodynia and thermal hyperalgesia was performed on days -1, 2, 6, 10 and 14. The results were analysis by mixed mode3l ANOVA (P<0.05).Results & Conclusion: The results of behavioral studies revealed that in MSCs group allodynia and hyperalgesia significantly decrease in comparison to DMED group, on day 6 after CCI and this effect was extending until day 14. It seems that preemptive injection of MSCssupernatant can be effective in prevention of neuropathic pain in CCI induced neuropathic pain in rat.