the effects of progesterone and 4-methylcatechol on sciatic nerve structure and function in rats with diabetic neuropathy

Background and Aim : diabetic neuropathy is one of the most common complications affecting more than 50-60% of diabetic patients and it is a common cause of non-treatment amputation and autonomic failure the aim of this study was desighed to investigate the possible beneficial effects of progestron (p) and 4-methylcatechol(4mc) on histopathological changes of sciatic nerves in streptozotocin-induced diabetic ratsMethods : the adult male rats(spargue-dawley) were randomly divided into one of five groups: co(control nondiabetic ), dm(untreated diabetic), dmp(diabetic treated with p), dmp4mc(diabetic treated with 4mc) and dmp4mc( diabetic treated with p+4mc), each group contain15 animals. diabetes was induced in animals by a single dose injection of streptozotocin ( stz, 55 mg, i.p). four weeks after the stz neuropathy had been stablished, rats in dmp group were treated with p(8 mg/kg,i.p), in dm4mc group with 4mc(10 mg/kg, i.p.). and in dmp4mc group with p+ 4mc every two days for 6 weekResults : diabetic rat showed a significant reduction in mean myelinated and unmyelinated fibers diameter,axon diameter and myelin sheath thickness in the sciatic nerve after 6 weeks. in sciatic nerve of the untreted diabetic group was observed endoneurial edema and were also increased numbers of myelinated fibers with infolding into the axoplasm , irregularity of fibers, myelin sheath with unclear boundaries and alternation in myelin compaction . in diabetic treatment with 4mc prevented all these abnormalitties in treated diabetic rats.an increased of pmp22 expresion expression and ngf level in sciatic nerve also was seen in the administration of prog and 4mcConclusion : our finding suggest that 4mc as a tharapetic approach can protect , histomorphological and ultrastructural changes and increased ngf and pmp22 level in sciatic nerve after the establishment of peripheral diabetic neuropathy.

کلیدواژه ها:

progestrone ، 4-methylcatechol ، sciatic nerve