The Effect of Embryonic Cerebrospinal Fluid on GFAP Expression of Epidermal Neural Crest Stem Cells

سال انتشار: 1398
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 409

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شناسه ملی سند علمی:

NSCMED08_483

تاریخ نمایه سازی: 15 دی 1398

چکیده مقاله:

Background and Aim : Embryonic cerebrospinal fluid (e-CSF) secretes from choroid plexuses into the brain ventricle. This fluid due to presence of growth factors has potential proliferative and differentiative effects. Epidermal neural crest stem cells (EPI-NCSCs) are multipotent stem cells which were located in bulge region of adult hair follicles. In this study, the effect of e-CSF on expression of GFAP marker was investigated.Methods : e-CSF was collected from cisterna magna of embryonic 17, 18, 19 and 20 old-day Wistar rat embryos. The EPI-NCSCs was obtained from hair follicle bulge region of two-months male Wistar rat’s vibrissa. These cells were cultured in medium containing α-MEM, 10% FBS and 5% CEE in presence of 1% antibiotic. Then, CSF of E17-E20 was added to the media with 10% ratio(v/v). The expression of GFAP marker was evaluated by immunocytochemistry.Results : The results showed that E17-E20 CSF causes considerable induction of neural-like morphological changes in EPI-NCSCs comparing with controls. The results also showed the expression of GFAP marker in cells treated with e-CSF comparing to the controls.Conclusion : The results demonstrate the differentiational influence of embryonic cerebrospinal fluid and also differentiational potential of epidermal neural crest stem cells. Our results show that e-CSF induce GFAP expression in EPI-NCSCs. Due to their high degree of inherent plasticity and their easy accessibility, EPI-NCSCs are promising candidates for cell-based therapy.

کلیدواژه ها:

نویسندگان

Alireza Sahebi

Department of Cell and Molecular Biology, Faculty of Biological Sciences, Kharazmi University, Tehran

Mohammad Nabiuni

Department of Cell and Molecular Biology, Faculty of Biological Sciences, Kharazmi University, Tehran

Kazem Parivar

Department of Biology, Sciences and Research Branch, Islamic Azad University, Tehran

Siamak Yari

Department of Biology, Faculty of Sciences, Bu-Ali Sina University, Hamedan

Sareh Pandamouz

Department of Cell and Molecular Biology, Faculty of Biological Sciences, Kharazmi University, Tehran

Mohammad Hossein Mohammadi

Department of Cell and Molecular Biology, Faculty of Biological Sciences, Kharazmi University, Tehran