Effects of Cyperus Rotundus Extract on Spatial Memory impairment, Alzheimer s disease and Neuronal differentiation in Beta Amyloid Rat Model

Background and Aim : Alzheimer s disease (AD) is one of the most common neurodegenerative diseases in the population older than 60 years and characterized by, progressive memory and cognitive impairment. Cyperus rotundus, a traditional medicinal herb, has analgesic, sedative, and anti-inflammatory effects. This plant is used to increase memory in Islamic traditional medicine and Arab countries especially in neighboring countries such as Iraq. This study was designed to consider the effects of Cyperus rotundus (C.rotundus(CR)) extract on memory impairment and neurogenesis in Beta Amyloid rats model.Methods : Materials and methods: In this study 42 Male Wistar rats were randomly divided into six groups (n= 7) for evaluation of baseline training performance in the Morris water maze test. Then amyloid-beta (Aβ1-42) was injected in animal hippocampal CA1 bilaterally in four groups. The first probeee trial was performed 21 days after Aβ injection, then, the C.rotundus extract in three different doses of 250, 500, and 750 mg/kg were administered to three Aβ injected groups for one month, then after that the second probeee trial was performed and rats were sacrificed after 28 days of the second probeee trial. The neurogenesis was detected in the hippocampus, by immunohistochemical staining.Results : This study showed that spatial memory increased in a behavioral test in AD treated group with C. rotundus extract, compared with the AD group not received our extract (p=0.02). Also, immunohistochemical staining revealed that neuronal differentiation have been occurred in dentate gyrus of the hippocampus in AD treated group with C. rotundus extract compared with the AD group (p=0.01).Conclusion : : This study showed that C. rotundus extract, repaired spatial memory impairment in the Aβ rat model, through increased neurogenesis in the hippocampus, which could be related to the flavonoid components in the extract.

کلیدواژه ها:

Cyperus Rotundus ، Spatial Memory ، Neuronal differentiation