Short-Term Stimulation of Stem Cells with Various Osteogenic Factors for Enhanced Differentiation Into Bone

Background and Aim: A one-step concept for bone regeneration has beenpostulated in which human adipose stem cells (hASCs) are harvested,triggered to differentiate, seeded on carriers, and implanted in the sameoperative procedure. Toward this goal it was investigated whether short(minutes) incubation with osteogenic factors like BMP-2 and polyaminessuffices to kick-start differentiation of hASCs seeded on calciumphosphate carriers towards the bone cell lineage.Methods: hASCs were treated with or without physiological dosages ofthe various factors for 10-30 minutes, and seeded on calcium phosphatebone substitutes. The attachment was determined after 30 minutes.Proliferation (DNA content) and osteogenic differentiation (alkalinephosphatase activity, gene expression) were analyzed for up to 3 weeksof culture.Results: We found that markers for early and late bone cell differentiation,e.g. gene expression of runx-2, collagen-I, osteonectin, osteocalcin, andosteopontin, as well as alkaline phosphatase activity were increased byshort osteogenic factor pre-incubation.Conclusion: It can be concluded that short osteogenic factor preincubationof hASCs seeded on bone substitutes had a long-lasting stimulating effecton osteogenic differentiation in vitro. These results strongly support aone-step clinical concept for bone regeneration.

کلیدواژه ها:

Adipose stem cells ، Osteogenic factors ، Osteogenic differentiation ، Bone regeneration