The Effect of Amniotic Membrane-Derived Mesenchymal Cells Transplantation on Cardiac Function Improvement in a Model of Heart Failure in Male Rats Through the Effect on The Expression of bcl2, and bax

Background and Aim: Heart failure is an increasing and debilitatingproblem throughout the world. Currently, cell therapy is a beneficialstrategy compared to previous treatments with the potential to replaceinjured myocardial tissue. It seems that using mesenchymal stem cellsderived from the amniotic membrane is an appropriate therapeuticmethod for heart failure.Methods: Thirty-five male Wistar rats were randomly assigned into 5groups (7 in each group). Study groups were: 1. Control group, 2. Heartfailure group (HF), 3. Sham group, 4. Culture media group, 5. Stemcells transplantation group (SCT). Heart failure model was inducedby subcutaneous injection of 170 mg/kg/d isoproterenol during 4consecutive days and the induction was confirmed by echocardiography28 days later. In stem cells transplantation group, 3×106 cells weretransplanted into myocardial tissue in a 150 μL culture media. By use ofechocardiography, cardiac function was examined. Western blot methodwas used to determine the expression of bcl2, bax and p53 proteins.Results: Echocardiography parameters showed that ejection fraction inthe HF group increased from 58.73± 0.9 to 81.25± 0.5 in the SCT groupwhich indicates 22.52 change in this parameter. Fractional shorteningincreased from 27.8±53.58 in HF group to 45.6±55.91 in the SCTgroup indicating a change of 22.52. Protein level measurements showedincreased levels of p53 and bax proteins in the HF group.Conclusion: It seems that amniotic membrane-derived mesenchymalstem cells transplantation significantly improves the cardiac functionin heart failure through effects on the expression of bcl2, bax and p53proteins.

کلیدواژه ها:

Heart failure ، Amniotic membrane-derived mesenchymal stem cells ، Ejection fraction ، Fractional shortening ، p53 ، bcl2 ، bax