The Stability of Noncryopreserved Mesenchymal Stem Cells

سال انتشار: 1397
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 287

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شناسه ملی سند علمی:

NSCMRMED03_131

تاریخ نمایه سازی: 30 دی 1397

چکیده مقاله:

Background and Aim: Mesenchymal stem cells (MSCs) use, due to theirmultipotency, low immunogenicity, and tissue repair ability is growingrapidly. A challenge in MSCs therapy is that the bench to the bedtime offreshly cultured MSCs cannot exceed 24 hours. For use, after 24 hours,we need to cryopreserve MSCs. Cryopreservation can preserve the cellsfor years, but it is a costly and damaging process. Here we introduce amethod to extend the bench to bed lifetime of MSCs up to 3 to 5 dayswithout the high cost and cell-damaging effects of cryopreservation. Ourmethod is based on preserving the MSCs in human AB+ plasma.Methods: MSCs of 10 tissue samples - 3 adipose, 3 bone marrow, and4 Wharton s jelly - were cultured and expanded in standard conditions.After the cell harvest, 25 million MSCs of each sample were suspendedin human AB+ plasma at 2×106 cell/mL density. Afterward, the cellsuspension was equally divided into 12 sterile capped tubes. Sixtubes were put in a fridge at 5±3°C and the other 6 were left in roomtemperature (22±3°C). During the next 6 days, each day, two tubes (onefrom each group) were picked to assess the viability and ability to thegrowth of the MSCs. For the tubes picked on day 3, we also assessedthe differentiation potential to the adipocyte and osteocyte of the MSCs.The results were analyzed by computing the means over 10 samples andapplying the independent-samples t test to those means.Results: The sample means for both cell expansion ability and cellviability for 1 to 6 days storage length were compared between the two fridge and room temperature groups. No significant differencesbetween the two groups for any of the storage lengths were observed(independent t test). We found that more than 80% of the cells remainedalive up to 6 days of storage in both groups. We observed a gradualdecrease in cell growth potential from 1 day stored samples to 6 daysstored samples in both groups. However, the cell confluency percentagereached 80% under 20 days for all samples except the 6 days storedones. No significant differences were observed between the two groups.The differentiation potential to adipocyte and osteocyte which weretested in the 3 days stored tubes were positive in all samples.Conclusion: This study showed that we can store the MSCs in humanAB+ plasma at least for 3 days (probably up to 5 days) in both room andrefrigerator temperatures without losing their stemness characteristic.Based on the available equipment (coolers and refrigerators), we suggest5±3°C as a storage temperature for management reasons. Moreover,assessing the differentiation potential of the MSCs in 5 days storedsamples may prove that this method can be used for storing the cells upto 5 days.

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نویسندگان

Mandana Mohyeddin Bonab

Sinacell Research and Product Company, Tehran, Iran

Azam Abedi

Sinacell Research and Product Company, Tehran, Iran

Fahime Anici

Sinacell Research and Product Company, Tehran, Iran

Ronaz Haghshenas

Sinacell Research and Product Company, Tehran, Iran