Evaluation of the Effect of BMSCs Condition Media and Methylprednisolone in TGF-β Expression and Functional Recovery AfterAcute Spinal Cord Injury

Background and Aim: Inflammation is one of the most importantcontributing factors to the spinal cord after injury. It makes causingMacrophages and other inflammatory agents to attack the site of thelesion. One of the most important ways to control inflammation is usingMethylprednisolone (MN) and Bone Marrow Stromal Cells ConditionMedia (BMSC-CM). They can reduce inflammation and improvefunctional recovery after spinal cord injury.Methods: BMSCs were analyzed by flow cytometry for mesenchymalmarkers. After acute spinal cord injury, MN and BMSC-CM were injectedintraperitoneally and the recovery rate was evaluated by BBB and narrowbeam test.Results: There were significant differences in functional recovery betweenMN+CM group Relative to other groups. TGF-β1 expression decrease inCM+MP group Compared to the control group.Conclusion: Simultaneous use of MN and BMSC-CM will improverecovery from spinal cord injury, reduce inflammation and improvefunctional recovery.

کلیدواژه ها:

Spinal cord injury ، TGF-β1 ، Inflammation ، BMSC ، Methylprednisolone