The Odontogenic Differentiation of Human Dental Pulp Stem Cells on Hydroxyapatite-Coated Biodegradable Nanofibrous Scaffolds

Background and Aim: The authors aimed to design nanofibrous (NF)scaffolds that facilitate odontogenic and osteogenic differentiation ofhuman dental pulp-derived mesenchymal stem cells (DPSCs) in vitro.Methods: For this purpose, hydroxyapatite (HA)-loaded poly (L-lacticacid)/poly (ɛ-caprolactone) (PLLA: PCL 2; 1) blend NFs were preparedusing the electrospinning method. Alizarin red activity and cell viabilitywere evaluated by MTT assay, and SEM revealed the proliferationproperties of NF scaffolds.Results: Optimized nanofibers PCL/PLA/HA (PCL/PLA,2:1 w:w) of variousHA contents with average diameters of 100-300 nm were obtained usingthe electrospinning method. QRT-PCR results demonstrated that HAloadedPLLA/PCL can lead to osteoblast/odontoblast differentiation inDPSCs through the up-regulation of related genes, thus indicating thatelectrospun biodegradable PCL/PLA/HA has remarkable prospects asscaffolds for bone and tooth tissue engineering.Conclusion: The attachment, proliferation, and differentiation behaviorsof the osteoblast-like cells on the PCL/PLA and PCL/PLA/HA nanofibrousmats were fully investigated to assess their potential application asscaffolds for bone/dentin tissue engineering. HA-coated nanofiberscaffolds clearly displayed a positive effect on the differentiation ofDPSCs into osteogenic/odontogenic cells and moderate effect on cellproliferation.

کلیدواژه ها:

Dental pulp stem cells ، Differentiation ، Nanofibers ، Odontogenic ، Regenerative medicine ، Scaffold ، Tissue engineering