Fibrin Scaffold as Substrate for Culture of Bone Marrow Mesenchymal Stem Cells

Background and Aim: Mesenchymal Stem Cells (MSCs) are knownas appropriate cells in regenerative medicine. Applications of MSCsin clinical practice and regenerative medicine need to keep MSCs aspluripotency stem cells. In this study, the effect of fibrin scaffold onstemness gene expression of MSCs was examined.Methods: Bone marrow-derived MSCs were cultured in tissue cultureplates (2D) and 3-dimensional (3D) fibrin scaffolds. The effect of fibrinscaffold on the proliferation of MSCs was evaluated by MTT assay.Stemness state was evaluated by qRT-PCR for OCT4, SOX2 genes, andflow cytometry for Nanog protein.Results: Cultured MSCs on fibrin scaffold were able to proliferateaccording to data obtained by MTT assay. Gene expression of OCT4 andSOX2 had a significant increase in cells were cultured in the 3D conditionin compared to 2D condition (P<0.05). Also, the higher expression ofNanog protein in 3D culture was observed (P<0.05).Conclusion: Thorough evaluation of important pluripotency regulatorssuch as OCT4, SOX2, and Nanog; we demonstrated that fibrin scaffold could maintain MSCs in a stemness state.

کلیدواژه ها:

Mesenchymal stem cells ، Stemness ، Fibrin scaffold ، OCT4 ، SOX2 ، Nanog