Choosing the Right Source for Induction of Pluripotent Stem Cells for Cell Therapy Proposes

Background and Aim: IPSCs are the cells with the potential to differentiateto all of the three embryonic layers. Although many attempts have beenmade to differentiate these cells to a specific type, many of them maybe hindered by many factors such as apoptosis, partial reprogramming,cancer consideration, and etc. One of the best ways to overcome thisproblem is finding a new source of somatic cells which have enoughpotential to differentiate with optimal conduction to needed cells.Methods: We analyze the NGS FASTA files of 27 samples from IPSC withfibroblast, hepatocytes, melanoma, mesenchymal source and embryonic cell line and embryonic body with gene pattern and GEO softwarepackage. Their differential signature has been identified and heat mapshave been established for a comprehensive and comparable search. Afterthis stage, we have picked the subsets of genes with a p-value under0.05 and their networks have been established by gorilla and Reactomepackage. We compared the results to selecting the right source for celltherapy proposes.Results: The results show us the IPSC with fibroblast cells have been thelowest amount of catabolic and metabolic in lipids, amino acids, andglycolysis pathways differences with normal embryonic stem cells. It isnoteworthy that changing in this pathway is the main step in the formationof cancer stem cells. This data shows us that fibroblast cells are the safestsource for using for cell therapy and clinical usages. Our data indicateIPS cells with hepatocytes source are prone to differentiate to beta cells,these cells have an upregulation in secretion and insulin-related pathwayand they preserve their protein localization and structural pathwaywhich are an important condition for tissue engineering and engraftingconcerns.Conclusion: Based on these finding we can conclude that fibroblast cellsgenerally can be considered as the safest source for induction towardpluripotency, this is an important consideration as it has been seen interatoma tests. Another finding of ours is, IPSC with a hepatocyte sourceshow similar pattern to stem cell progenitor of the pancreas and theyare a great source in regenerative medicine for diabetic and pancreaticrelated diseases.

کلیدواژه ها:

Pluripotent stem cells ، cancer cell therapy