Investigation of the Relationship Between Mir129a and Mir149 with the Ability to Differentiate Umbilical Stem Cells Towards

Background and Aim: Micro-RNAs are small non-coding RNAs that areabout 19 to 24 nucleotides long and have an effect on RNA inhibition,post-transcriptional expression, and gene expression. Micro-RNAscount for about 3 percent of the total human genome and RNAs play animportant role. In the regulation of immunological functions, includingacquired immune responses, the development, and differentiation ofimmune cells and self-healing. These small regulators of expression ofgenes have specific patterns for expression, such as a specific patternof expression in particular tissues, as well as in terms of differentiation,illness.Methods: Separation of CD133+ stem cells by MACS was performed.After 9 days, the tendency towards T lymphocytes with CD4 and CD8markers was performed by flow cytometry. Real-time PCR and RT-PCRwere used for the analysis of microRNAs expression. Finally, the datawere analyzed by SPSSV.24 software.Results: The results showed that expression of Mir129a with a mean of5823 with a standard deviation of 395 and Mir149 with a mean of 5769with a standard deviation of 162 in treated cells was higher than thatof non-transfected cells. Also, the expression level of CD4 markers andCD8 in hematopoietic stem cells that are adjacent to the virus lacking thedesired fragment is much less than cells treated with these two markers.Conclusion: Based on these results, with the increased binding ofMir129a and Mir149, the ability to differentiate to T lymphocytes in theCD133 hematopoietic stem cells isolated from the umbilical cord bloodis greater.

کلیدواژه ها:

Mir129a ، Mir149 ، T-lymphocyte ، Stem Cell ، Umbilical cord