Novel Engineering-Based Methods for Tissue Growth Assessment in Lung Regeneration

Background and Aim: Lung tissue engineering is a promising field withthe goal of developing functional substitutable tissues for transplantationor in vitro monitoring of drug toxicity in model disease tissues. Toobtain efficient engineered tissues, monitoring cell viability is one ofthe significant difficulties that should be overcome. This study aimed toestablish monitoring strategies which were required to assess the effectof different stimuli and feeding and aeration strategies on cell survivaland growth.Methods: In this research, two different monitoring systems weredeveloped based on respiration activity. Human umbilical cord veinendothelial cells (HUVECs) were seeded into rat acellular lung scaffolds,and then, cell viability was monitored during the culture period (3-dayand 10-day periods).Results: The monitoring systems provided cell growth profilesrepresenting essential information on cell viability and growth statesduring the culture period. MTT analysis, H&E staining, SEM imaging,and glucose consumption measurement proved the consistency of theresults obtained by the monitoring systems.Conclusion: The monitoring strategies provided useful information whichhelps control, optimize, and modify the lung regeneration process andeventually produces a functional implantable lung.

کلیدواژه ها:

Acellular lungs ، Monitoring strategy ، Tissue engineering ، Regenerative medicine ، Lung regeneration