Improvement of Diabetic Open Skin Wound Healing by Human Bone Marrow-Mesenchymal Stem Cells Conditioned Media: APreclinical Study

Background and Aim: Wound healing is a complex process that isimpaired in diabetic patients due to several factors. So far, the positiveeffects of mesenchymal stem cells secretome in the wound healingprocess have been reported. In this study, we investigated the effect ofhuman bone marrow mesenchymal stem cells conditioned media (hBMMSC-CM) on gene expression of some effective factors involved inwound healing and the strength of healed wound in diabetic rats.Methods: The animals were divided into the normal wound (NW),diabetic wound (DW) and diabetic wound treated by the condition media(DW-CM) groups. Diabetes was induced by Alloxan. A full skin thicknesswound was created on the back of the rats. Then, the conditionedmedium was prepared from hBM-MSC and DW-CM rats received 200microliters of the CM intravenously. Then, the wounds were sampled andexpression of KGF and TGF-β1 genes was examined by RT-PCR on days4 and 7 after wounding. Also, on the 15th day, a standard sample fromeach healing wound was submitted for the biomechanical examinationusing tensiometric study.Results: There were no significant differences between DW-CM and DWgroups in the term of KGF gene expression at 4th and 7th days. While expression of the TGF-β1 gene in DW-CM group decreased significantly(P<0.05) compared to DW group at 4th day. Furthermore, biomechanicalparameters of the healing wounds in the DW-CM group significantly(P<0.05) increased in comparison to the DW group.Conclusion: It seems that using the conditioned medium derived fromhuman bone marrow mesenchymal stem cells positively affects theexpression of inflammatory factors involved in wound healing, and leadsto improvement of biomechanical parameters of the healed wound in thediabetic condition.

کلیدواژه ها:

Mesenchymal stem cells ، Conditioned medium ، Diabetic wound healing ، Gene expression ، Tensiometry