Menstrual Blood Stromal Stem Cells: An Optimized Protocol for Isolation and Proliferation

Background and Aim: stem cells are frequently used in various medicaltherapies and have also been proposed as promising candidates for futuretherapies. Recently, numerous studies have been demonstrated that stemcell-based therapy has a great potential in a wide range of diseasessuch as fertility. However, Cell therapy is found in the early stages fortreatment of female infertility. Stromal stem cells can be consideredas one of the commonly used stem cells in cell therapy researches,which have prominent characteristics, including highly proliferativeand differentiated powers, along with immunosuppressive properties.Menstrual blood stromal stem cells (MBSCs) are considered as a novelsource of endometrial stem cells (EnSC) which their therapeutic potentialwas verified in several studies. The choice of a suitable cell source anddevelop and optimize its own isolation and expansion protocol, are thefirst step in the initiation of cell therapy.Methods: A human EnSC line was derived from the menstrual bloodof 30-40 years old infertility women such as endometriosis, polycysticovary (PCO), premenopausal menopausal syndrome (POF) andidiopathic were selected as four experimental groups. Menstrual bloodwas collected during the second or third days of menstruation with IUIcatheter. Mononuclear cells were separated by Ficoll-Paque densitygradientcentrifugation. Cells were then expanded in DMEM mediumcontaining 10% FBS, 1% penicillin/streptomycin.Results: EnSCs isolated from the women showed fibroblast-like cellsmorphology. The isolated EnSCs were propagated in vitro and passageswith excellent cell viability.Conclusion: In general, our findings suggest that the present protocolcan be used to isolate stem cells derived from the menstrual blood ofinfertile patients, in order to of differentiating into oocyte-like cells, andeventually introducing an innovative cell-based therapy for infertilewomen. In addition, this protocol is a very simple, non-invasive, lowcost, short period, and high-efficiency method, which can be usedfor clinical and in vitro research. However, part of this research is stillunderway and will continue to differentiate to oocyte-like cells.

کلیدواژه ها:

Stromal stem cells ، Optimization ، Infertility women ، Menstrual