Effect of Elaeagnus Angustifolia Extract in Bone Tissue Engineering Nanofiber Technology

Background and Aim: Modern societies tend to use medicinal plantsfor their basic health care needs. Elaeagnus angustifolia is one of the medicinal herbs with antinociceptive, anti-inflammatory, antibacterialand antioxidant properties widely used in the treatment of patients withrheumatoid arthritis and osteoarthritis symptoms.Methods: In this work, for the first time, E. angustifolia extract was loadedon PCL-PEG-PCL nanofibers and studied their potential application inbone tissue engineering. Fourier transform infrared spectroscopy (FT-IR),field emission scanning electron microscope (FE-SEM), contact angle andmechanical test were applied to evaluate the morphology and chemicalproperties of nanofibers. FE-SEM images showed that all samples hadbead-free morphologies with average diameters ranging from 100-200nm. The cell response to the E. angustifolia extract loaded PCL-PEG-PCLnanofibers was evaluated by means of human dental pulp stem cells(hDPSCs).Results: hDPSCs showed improved adhesion and proliferation capacityon the E. angustifolia loaded nanofibers compare to pristine PCL-PEGPCLnanofiber. Alizarin red S assay and alkaline phosphatase activityconfirmed the nanofibrous scaffolds could induce the osteoblasticperformance of hDPSCs (P<0.05). QRT-PCR results confirmed that E.angustifolia loaded nanofibrous scaffolds significantly upregulated thegene expression correlated to osteogenic differentiation (P<0.05).Conclusion: The results suggested that E. angustifolia extract loaded withPCL-PEG-PCL nanofibers might have potential applications for bonetissue engineering.

کلیدواژه ها:

Elaeagnus angustifolia ، Scaffold ، Electrospinning ، Human dental pulp stem cell ، Tissue engineering