Medium containing different concentrations of catalase as a strategy for optimizing sperm parameters and chromatin in normospermic persons

Background: Aerobic metabolism of human sperm pro-duces reactive oxygen species (ROS). During sperm preparation. antioxidant remove from semen This damage. change in the sperm motility and DNA damage. ontrol of lipid peroxidation is exerted by antioxidant. one of important antioxidant is catalase. Studies show that the addition of catalase to the sperm media effect on sperm function.Objective: Therefore, the aim of this choose the best concentration of catalase for optimizing sperm parameters and chromatin in normospermic persons.Materials and Methods: Semen samples were selected from normozoospermic men. The semen of each person was divided into eight groups: one group as control and seven as treatment groups. Spermatozoa were prepared by swim-up method for each group.In the treatment groups, different concentrations of catalase (200, 150, 100, 50, 10, 1 and 0.1 IU/mL) were added. In all groups the semen was incubated for 1 hr. After incubation, percentage of motility, viability and morphology of spermatozoa as well as chromatin quality were assessed for all groups. After this time, these tubes were incubated at room temperature. The sperm motility was only analyzed after 4 and 24 hr.Results: Sperm motility in one hour after exposure to different concentrations of catalase showed no significant difference between groups. However, those spermatozoa exposed to 100 IU/ml of enzyme had the highest motility (71%±8.431 vs. 58%±10.03, p=0.041). Sperm motility after 4 and 24 of exposure to 100 IU/ml of enzyme at room temperature was similar to one hour. However, chromatin quality had the best condition in low concentration of catalase (p=0.015).Conclusion: This study showed that concentration of 1 IU/ml of catalase should be used to improve parameters and sperm chromatin because at low concentrations, sperm histone replacement by protamines may improve sperm motility in addition to protecting sperm DNA from damage. Therefore, we recommend adding low concentration of catalase (1 IU/ml) to the sperm medium to optimize their movement and protect chromatin structure and integrity.

کلیدواژه ها:

Catalase ، Chromatin ، Motility ، Sperm ، Viability