The effect of medium on the rate of chromosomal instability in human amniotic fluid cells

سال انتشار: 1398
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 374

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شناسه ملی سند علمی:

RMED08_085

تاریخ نمایه سازی: 21 مرداد 1398

چکیده مقاله:

Background: Recent developments in regenerative medicine have led to the search for stable, safe and highly accessible stem cell sources with therapeutic potential. Although the genetic stability of stem cells to be used in cell therapy and other clinical applications is strongly critical, but the relationship between in vitro expansion of stem cell lines and their genomic stability has not yet been completely elucidated.Objective: The present paper aims to validate the possible role of medium on the rate of chromosomal instability in human amniocytes until development of replicative senescence.Materials and Methods: Amniotic fluid samples were donated by genetics laboratories of Yazd reproductive sciences institute. We received the samples at first passage when amniocentesis procedure had been over. Each sample was cultured with two different medium: 1) DMEM supplemented by 4 mM L-Glu, 10 mM HEPES, 15% FBS, 1% PenStrp; 2) AmnioMAX II supplemented by 10 mM HEPES and 1% PenStrp. The samples were harvested between passages 4-9 when they had been senescent and not be able to be passaged anymore. Afterwards, the cells were fixed on slide and evaluated considering eight probes for X-13/18/21 and Y-15/16/22 chromosomes by the FISH technique.Results: Our results showed that total chromosomal abnormality around 9.9% and 4.3% in DMEM and AMX respectively. Investigating 535 and 510 cells in samples from two medium showed overall 75 abnormal cells more with polyploidy.Conclusion: Overall, according to potential of hAFCs and derived- stem cells and regarding to low frequency of chromosomal abnormalities and high stability of them in long term cultures, we would suggest applying them in cell therapy and regenerative medicine research.

نویسندگان

SM Hoseini

Biotechnology Research Center, International Campus, Shahid Sadoughi University of Medical Sciences, Yazd, Iran

F Montazeri

Abortion Research Center, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical, Yazd, Iran

H Heidarian Meimandi

Abortion Research Center, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical, Yazd, Iran

F Zarein

Abortion Research Center, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical, Yazd, Iran

SM Kalantar

Abortion Research Center, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical, Yazd, Iran