MSOME detected fine sperm abnormalities in infertile men with high BMI

Materials and Methods: A total of 76 men presented as male factor with average age of 34.4±6.1. They were categorized in 3 group of men I: normal weight (BMI: 18.5-24.9 kg/m2), II: overweight (BMI: 25-29.9 kg/m2), III: obese (BMI: ≥30 kg/m2). MSOME criteria were compared between 3 groups as the presence and size of vacuoles (none, small, medium, large, mix), head size (normal, small, large), Presence or absence of cytoplasmic droplets; head shape (normal or abnormal) and acrosome status. Sperm parameters were assessed according WHO criteria. Protamine deficiency was also assessed in sperm chromatin by chromomycin A3 (CMA3) staining.Results: There were significant differences between groups II and III for large head size (p=0.028) but rate of cytoplasmic droplets, head shape, acrosome status and sperm parameters were no difference in 3 groups. Also, the data showed a significant correlation between BMI with large vacuoles in spermatozoa (r=0.23, p=0.043) and large head size (r=0.24, p=0.036). High level of BMI had a negative correlation with sperm motility, but it was insignificant (r=-0.039, p=0.74). Sperm chromatin status in 3 groups were similar (p=0.56).Conclusion: Obesity is associated with both large vacuoles and large head size in spermatozoa in infertile men. MSOME, is a useful tool for accurate selection of spermatozoa for ICSI program.

کلیدواژه ها:

Body mass index ، Male infertility ، Obesity ، MSOME criteria