The effect of Inofolic on intracellular reactive oxygen species (ROS) and reduced glutathione (GSH) levels in the MII oocyte of DHEA-induced PCOs mice

Background: Polycystic ovary syndrome (PCOS) is associated with hyperandrogenism, polycystic ovaries and ultimately oocytes whit poor quality. The administration of myo-inositol was associated with a decreased of serum testosterone, increase insulin sensitivity and improves the oocytes quality.Objective: The purpose of this study is to determine the effects of inofolic on oocyte ROS and GLUT levels in PCOs model.Materials and Methods: The present study was performed in DHEA-induced mice. Female NMRI mice were treated with a vehicle control (Sesame Oil 0.05 ml and saline) or DHEA (6 mg /100 g body weight) or DHEA plus inofolic (37 mg /100g body weight) for 20 consecutive days. After 20 days superovulation was performed, Mature oocysts (MII) were retrieved from isolated ovaries for determination of intracellular reactive oxygen species (ROS) and reduced glutathione (GSH) levels.Results: Respectively GSH and ROS were significantly lower and higher in DHEA-treated oocytes compared with vehicle-treated. In DHEA+inofolic group, ROS was significantly reduced in contrast to the DHEA group and, On the contrary, GLUT has risen. Conclusion: It seems that the inofolic plays an important role in reducing ROS and increasing the antioxidant capacity of mice oocytes in the PCOS model.

کلیدواژه ها:

Inofolic ، Oocyte ، Reactive oxygen species ، Reduced glutathione ، PCOS mice model