Evaluation of semen sampling container toxicity with human sperm motility assay and mouse embryo assay

Background: To improve the success rate in the ART lab, all consumables material and containers should be evaluated in quality control aspect. In this regard, two methods, Human sperm motility assay (HAS) and mouse embryo assay (MEA) are used for assessment of semen sampling containers toxicity.Objective: Assessment of semen sampling containers toxicity with Human sperm motility assay and mouse embryo assay.Materials and Methods: In this study, both MEA and HSA tests were used to evaluate the toxicity of sperm sampling containers. To carry out the HSA test, after the swim up of the normozoospermic samples (which had a motility more than 50%) was subjected to sampling containers for 1 hour. Both control and experimental groups were evaluated after 30 minutes, 1, 2, 4 and 24 hours. in each group, the percentage of sperm motility was evaluated. The ration of sperm motility in the test group to the control group was considered as an indicator for toxicity. To carry out the MEA test, a GLOBAL culture medium, commonly used in the ART laboratory for embryo culture, was exposed to a sperm sample container for 1 hour. Then, the medium was transferred to the embryo culture dishes. In both experimental and control groups, Blastocyst formation was assessed after 24, 48, 72 and 96 hr. Both tests were performed triplicate.Results: The results of HSA test showed that the toxicity of the experimental group decreased significantly after 24 hours compared to the control group, while the results from MEA test showed that the toxicity level in the experimental group was not significantly different compared to control group.Conclusion: It seems that using semen sampling containers has less toxicity for sperm and embryo.

کلیدواژه ها:

Semen sampling container ، Toxicity ، Human sperm motility assay ، HSA ، mouse embryo assay ، MEA