Overexpression of c-Maf gene in women with endometriosis

سال انتشار: 1398
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 431

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شناسه ملی سند علمی:

RMED08_248

تاریخ نمایه سازی: 21 مرداد 1398

چکیده مقاله:

Background: Endometriosis is an inflammatory gynecologic disease in women of reproductive age. This disease is characterized as implantation and growth of endometrial tissues outside the uterine cavity. Women with endometriosis suffer from different signs and symptoms such as dysmenorrhea, chronic pelvic pain and infertility. The pathogenesis of endometriosis has not been completely understood. Several studies suggest that changes in immune responses in endometriotic milieus play an important role in pathogenesis of endometriosis. One of these alterations is change in different subsets of T helper (Th) cells. c-musculoaponeurotic fibrosarcoma (c-Maf) is a transcription factor that expressed in Th2, Th17 and T regulatory (Treg) which ultimately leads to the production of cytokines such as IL4, IL10 and IL21.Objective: The aim of this study was to investigate the expression level of c-Maf in the endometrial tissues of women with endometriosis compared to control ones.Materials and Methods: In this case control study, 13 women with endometriosis and 13 women without the endometriosis were enrolled in the study after diagnostic laparoscopy. Ectopic samples were collected through laparoscopic procedure and eutopic samples were collected using pipelle. The control endometrial samples were also obtained from normal endometrium by pipelle. The gene expression of c-Maf in normal, eutopic, and ectopic endometrial samples were evaluated quantitatively by real-time polymerase chain reaction (real time-PCR). Gene expression data were analyzed based on 2-ΔΔct to estimate the relative fold change values. Data were analyzed by one-way ANOVA followed by Tukey’s test. P<0.05 was considered as statistically significant. Results: Based on the findings of this study, the mRNA expression level of c-Maf was significantly increased in ectopic tissues of patients with endometriosis compared to eutopic and control endometrial tissues (p<0.05). Although mRNA expression of c-Maf was higher in eutopic endometrial tissues of endometriosis women compared to control ones but this difference was not statistically significant (p> 0.05).Conclusion: These data collectively identified that c-Maf overexpression may be critically involved in development of endometriosis beyond its role in promoting T helper responses. For getting more information, larger sample size is needed. Also expression studies of other transcription factors involved in T helper cell differentiation are recommended.

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نویسندگان

N Bajool

Department of Cell and Molecular Biology, College of BioScience, Islamic Azad University North Tehran Branch, Tehran, Iran

M Shahhoseini

Department of Genetics, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran

F Ramezanali

Department of Endocrinology and Female Infertility, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran.

E Amirchaghmaghi

Department of Regenerative Biomedicine, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran