Methylenetetrahydrofolate reductase C677T polymorphism and its association with recurrent pregnancy loss in South-Eastern Iranian population

سال انتشار: 1398
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 319

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شناسه ملی سند علمی:

RMED08_254

تاریخ نمایه سازی: 21 مرداد 1398

چکیده مقاله:

Background: An increasing number of studies have found that inherited thrombophilia can be associated with Recurrent Pregnancy Loss (RPL) in various populations all over the world. In this regard, one of the most studied polymorphisms is Methylenetetrahydrofolate Reductase (MTHFR) C677T which is suggested to be a prevalent etiology of RPL.Objective: This study aimed to investigate the association between C677T mutation and RPL in 370 women with idiopathic RPL and 220 women without history of abortion and at least 1 term pregnancy as the control group.Materials and Methods: Genomic DNA was extracted from peripheral blood using QIAamp DNA Blood Mini Kit (Qiagen). To identify C677T mutation, we performed the amplification refractory mutation system-polymerase chain reaction (ARMS-PCR) method. The frequency of mutation was statistically compared between two groups to examine whether significant differences exist between them.Results: Our results have shown the frequency of 24.9% in C677T mutation (heterozygote and homozygote) which was significantly higher in patients (p<0.05).Conclusion: As expected depending on the results of same investigation in other populations, MTHFR C677T mutation is responsible for a considerable increase in the risk of RPL.

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نویسندگان

SM Hoseini

Biotechnology Research Center, International Campus, Shahid Sadoughi University of Medical Sciences, Yazd, Iran- Khatam Al-anbia Superspecialty Clinic, Shahid Sadoughi University of Medical Sciences, Yazd, Iran

F Montazeri

Abortion Research Center, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran

E Hajimaghsoudi

Biotechnology Research Center, International Campus, Shahid Sadoughi University of Medical Sciences, Yazd, Iran- Khatam Al-anbia Superspecialty Clinic, Shahid Sadoughi University of Medical Sciences, Yazd, Iran

F Zarein

Abortion Research Center, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran