XO expression among healthy vs. oligospermia affected men before and after treatment with the palm pollen: in vivo research

Background: Exclusive cause of about 20% of couple infertilities is due to male abnormalities. In other 20-40% of the cases, the same issue is still a crucial role-player. On the other hand, oxidative-stress is considered as an important factor, deriving male infertility. There are confirmed evidence on phoenix dactylifera pollen (DPP) antioxidant properties, which are able to improve sperm quality. Xantin oxidase (XO) catalyzes xantin and hypoxantin oxidation in porin metabolism. This anzyme decreases molecular oxygen and culminate to a formation superoxide. Which will then play a role in sperm production through ROS.Objective: The objective of this study is to evaluate the effect of DPP on sperm parameters and expression of XO.Materials and Methods: 40 oligospermia affected and 10 healthy male individuals were studied. The affected group were treated for 40 consecutive days, Using gelatin capsules with 4 g DPP. Semen samples were obtained from all subjects both before and after the treatment. To assess sperm parameters, free 8-Isoprostane with Elisa method was tested as an index of DNA damage. mRNA expression of XO gene with real-time PCR method was tested in both stages. Data studied with statistical software SPSS.Results: XO expression was higher among the oligospermia affected group than the healthy control group. Infertile men treated with DPP had a significant reduction in the XO mRNA expression levels (p˂0.05). A similar reduction was also observed in free 8-Isoprostane. Besides, sperm parameters were improved (p˂0.05).Conclusion: These findings revealed that oxidative stress was truly a key factor involved in male infertility. DPP showed a positive effect on fertility factors and reducing sperm oxidative stress-related lethality. This herbal therapeutic agent can therefore be used as a low-risk therapeutic approach in treatment of male infertility.

کلیدواژه ها:

Infertility ، Oligospermia ، DPP ، XO