A Comparative Study of Tissue-Engineered Con-structs from Acropora and Porites Coral in a Large Animal Bone Defect Model

سال انتشار: 1398
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 347

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شناسه ملی سند علمی:

RROYAN20_015

تاریخ نمایه سازی: 29 مهر 1398

چکیده مقاله:

Background: To compare the therapeutic potential of tissue-engineered constructs (TECs) combining mesenchymal stem cells (MSCs) and coral granules from either Acropora or Porites to repair large bone defects.Materials and Methods: Bone marrow-derived, autologous MSCs were seeded on Acropora or Porites coral granules in a perfusion bioreactor. Acropora-TECs (n = 7), Porites-TECs (n = 6) and bone autografts (n = 2) were then implanted into 25 mm long metatarsal diaphyseal defects in sheep. Bimonthly ra-diographic follow-up was completed until killing four months post-operatively. Explants were subsequently processed for mi-croCT and histology to assess bone formation and coral biore-sorption. Statistical analyses comprised Mann-Whitney, t-test and Kruskal-Wallis tests. Data were expressed as mean and standard deviation.Results: A two-fold increase of newly formed bone volume was observed for Acropora-TECs when compared with Porites-TECs (14 sd 1089 mm3versus 782 sd 507 mm3; p = 0.09). Bone union was consistent with autograft (1960 sd 518 mm3). The kinetics of bioresorption and bioresorption rates at four months were different for Acropora-TECs and Porites-TECs (81% sd 5% versus 94% sd 6%; p = 0.04). In comparing the defects that healed with those that did not, we observed that, when major bioresorption of coral at two months occurs and a scaffold material bioresorption rate superior to 90% at four months is achieved, bone nonunion consistently occurred using coral-based TECs.Discussion: Bone regeneration in critical-size defects could be obtained with full bioresorption of the scaffold using coral-based TECs in a large animal model. The superior performance of Acropora-TECs brings us closer to a clinical application, probably because of more suitable bioresorption kinetics. How-ever, nonunion still occurred in nearly half of the bone defects