Breast Cancer Subtypes of Indonesian Patients based on Cd44/Cd24 Expression and Immunohistochemical Analysis

سال انتشار: 1398
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 378

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شناسه ملی سند علمی:

RROYAN20_032

تاریخ نمایه سازی: 29 مهر 1398

چکیده مقاله:

Background: This research was aimed to define characteristic of breast cancer subtypes based on CD44/CD24 expression us-ing flow cytometry analysis compared to ER, PR, HER2, Ki67, and P53 expression through immunohistochemistry assay.Materials and Methods: Breast cancer cells were isolated through enzymatic methods using proportional amount of Col-lagenase Type 1, Hyaluronidase, and Trypsin from BC tissue from 3 different patients. Flow cytometry analysis was per-formed to analyze the expression of CD44/CD24 markers in isolated breast cancer cells and Hs587t, SK-BR-3, MDA-MB 468, ZR-35-70-1 and MCF-7 cell lines used as comparative cells. Immunohistochemical analysis was conducted to exam-ine the expression of ER, PR, HER2, Ki67, and P53 in BC tis-sue sections.Results: We successfully isolated breast cancer cells from 3 different patients through enzymatic method. All isolated BC showed fibroblast cell-like morphology. Immunohistochemical analysis showed that BC patient 1, 2, and 3 classified as Lumi-nal A, Normal breast-like fibroadenoma, and Luminal A sub-type respectively. Isolated BC cells showed high proportion of CD44+/CD24- subpopulation (> 90% in patients 1 and 2; > 60% in patient 2). CD44+/CD24- has been reported to have progeni-tor/stem cells characteristics. However Luminal A and Normal breast-like fibroadenoma have the most favorable prognosis, the cell isolated from both subtypes contain CD44+/CD24- sub-population.Conclusion: The isolated BC cells from 3 different patients with different cancer subtype showed different pattern of CD44+/ CD24- expression. The highest subpopulation of isolated cells was CD44+/CD24-. These both different BC cell subtypes can be used as cell model for BC targeted therapy.

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نویسندگان

W Widowati

Faculty of Medicine, Maranatha Christian University, Bandung, Indonesia

D Jasaputra

Faculty of Medicine, Maranatha Christian University, Bandung, Indonesia

Y Heriady

Faculty of Medicine, Bandung Islamic University, Bandung, In-donesia

A Faried

Faculty of Medicine, University of Prima Indonesia, Medan, Indonesia