Nrf2 Pathway-Related Micrornas in Mesenchymal Stem Cell: New Approach in Regenerative Medicine

Background: In recent decades, with introducing of stem cells including mesenchymal stem cells (MSCs), huge body of medi-cal publications have focused on different applications of these mentioned useful cells in the field of regenerative medicine. However some limitations such as MSCs high death rate in vivo/ in vitro and susceptibility to oxidative stress are some of existing problems in the field of MSC-based cell therapy. Hence, application of practical and simple approaches to address these challenges are necessary. One of this strategy is manipulation of MSCs with cytoprotective gene, nuclear factor E2-related factor-2 (Nrf2). This study was aimed to evaluate Nrf2-related MicroRNAs expression in MSCs that might involve in oxida-tive stress response in these mentioned valuable cells.Materials and Methods: Umbilical cord MSCs were cultured in specific culture media and transfected with recombinant plas-mid construct containing Nrf2 using Fugene HP reagent. The expression level of some related MicroRNAs including miRNA 29a, miRNA 206, miRNA 34a and miRNA 153 was assayed using RT-PCR and Real time PCR in transfected MSCs. Non-transfected MSCs was considered as control.Results: RT-PCR and western blot confirmed Nrf2 expression in the transfected cells. Expression of miRNA 29a was up regu-lated in Nrf2-overexpressed MSCs compared with the control group. miRNA 206 was not expresseddi-erentially in both sub-jected groups. Transfected MSCs with ability to produce Nrf2 transcription and translation biomolecule showed decreased ex-pression levels of miRNA 34a and miRNA 153 in comparison with control.Conclusion: Manipulation of MSCs with Nrf2 down regulates the expression of some miRNAs, miRNA 34a and miRNA 153, that suppress its expression at translational level. This alteration in miRNAs expression might result in development of MSCs antioxidant capacities and prime them for harsh stress condi-tion.

کلیدواژه ها:

Mesenchymal Stem Cells ، Nrf2 ، Oxidative Stress ، MicroRNAs