Improvement of Optic Vesicle Formation during Retinal Organoid Development Using L-Ascorbic Acid

سال انتشار: 1398
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 378

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شناسه ملی سند علمی:

RROYAN20_046

تاریخ نمایه سازی: 29 مهر 1398

چکیده مقاله:

Background: The specific properties of pluripotent stem cells (PSCs) provide a suitable tool to study developmental events in vitro. Recent advances in 3D technology help to prepare a new aggregate formation method called organoid which mimics in vivo developmental events. Retinal organoid is one of the pio-neers organoid field. Recently numerous studies have offered to improve the first method of retinal organoid.Materials and Methods: In this study, by introducing the pro-pose factors into the first step of retinal organoid formation we modified the previous culture medium according to probable problems initiated by dissociation of human embryonic stem cells (hESCs).Results: According to our results, using L-ascorbic acid as an antioxidant agent and reducing knockout serum replacement (KSR) concentration, could increase the integrity of aggregates in comparison to the control group. We started a second me-dium containing fetal bovine serum (FBS), retinoic acid and N2 for one more week. Our data correspond to improve reti-nal organoid formation. The cells were organized into cyst-like structures with a columnar epithelium-lined lumen and also de-veloped the optic vesicle structure.Conclusion: Although a number of recent studies believe that despite optic vesicle some parts of body organoids express spe-cific markers of retinal differentiation, but the optic vesicle for-mation is still considered as a critical phenomenon for retinal development in vitro. Our results revealed that presence of anti-oxidant component can recover the individual hESC condition to further following stages and final results, and will facilitate an improved method for future developmental studies.

نویسندگان

S AVOJ

۱. Department of Biology, University of Payam Noor, Tehran, Iran. Department of Cellular Biotechnology, Cell Science Research Center, Royan Institute for Biotechnology, ACECR, Isfahan, Iran

F KARAMALI

Department of Cellular Biotechnology, Cell Science Research Center, Royan Institute for Biotechnology, ACECR, Isfahan, Iran

A KARIMI

Department of Biology, University of Payam Noor, Tehran, Iran

MH Nasr-Esfahani

Department of Cellular Biotechnology, Cell Science Research Center, Royan Institute for Biotechnology, ACECR, Isfahan, Iran