Mesenchymal Stem Cells Induced Early Apoptosis in B65-Neurogenic Differentiated Cells as In Vitro Cell Line Model for Alzheimers Disease

Background: Mesenchymal stem cells (MSCs) as undifferen-tiated multipotent cells are of particular interest due their po-tential clinical use in regenerative medicine. Stem cell-based therapies cast a new hope for neurodegenerative disease such as Alzheimer’s disease (AD) treatment as a replacement or re-generation strategy. Neurodegenerative diseases are groups of acute or/and chronic diseases that depending on operating con-dition cause to loss of neuroglia as well as neural cells. There is not absolute treatment due to the complete lack of understand-ing of the factors and conditions involved in them. Understand-ing the cellular mechanisms involved in Alzheimer s patients experience memory impairment is essential to design and de-termine the effectiveness of new drugs and therapies. The aim of this study is to explore the influence of secreted cytokines of MSCs on apoptosis of B65-neurogenic differentiated cell line as in vitro cell line model for AD.Materials and Methods: For this purpose, MSCs were isolated from bone marrow and co-cultured with B65-neurogenic dif-ferentiated cell (1:10) for 5 days. At the end of co-culture pe-riod, B65 differentiated cells were collected and subjected to Annexin/PI assessment.Results: It was shown that MSCs cause to significant induction of early apoptosis in B65-neurogenic differentiated cell. In de-tail, it was found that 37.3% of the cells were in early apoptotic stage (Annexin+, PI-), which was 2.91 times higher than that of the control group (12.8%).Conclusion: In this study, we reported that MSCS could be used for apoptosing B65-neurogenic differentiated cell as mod-el in cell-based therapy for AD.

کلیدواژه ها:

Mesenchymal Stem Cell ، B65-Neurogenic Differen-tiated Cells ، Alzheimers Disease