Effect of Cholestasis Syndrome on Proliferation Po-tential of Spermatogonial Stem Cells

سال انتشار: 1398
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 322

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شناسه ملی سند علمی:

RROYAN20_075

تاریخ نمایه سازی: 29 مهر 1398

چکیده مقاله:

Background: Cholestasis is a pathophysiological condition that is occurred due to various reasons such as infection, au-toimmunity, metabolic disorders or genetic factors. Histologi-cal studies have shown that cholestasis reduces proliferation, causes apoptosis, and prevents the maturation of germ cells. Spermatogonial stem cells (SSCs) are essential for self-renewal and differentiation during spermatogenesis. In current study we conducted a study to investigate the effect of cholestasis on the proliferation and viability of SSCs in the in vitro condition.Materials and Methods: Obstructive cholestasis was inducted in male Wistar rats by ligating the common bile duct. SSCs were isolated from healthy and cholestatic rats and the duration of colony appearance, the diameter of clones and the viability of SSCs were determined in culture medium. Briefly, the clones were fixed using paraformaldehyde 4% and subsequently, the number and diameter of the SSCs were determined under a microscope. To determine cell viability MTT assay was per-formed.Results: SSCs clones were observed in the control group after 2 days while they were appeared in the cholestatic group after 4 days. The results indicated that SSC had more proliferation in the control group within two weeks. There was also a significant difference between the diameter of the SSCs clones in the chol-estasis group compared to the control group and SSCs clones in the control group have been wider in two weeks. There was no significant difference in the survival of SSCs between two groups within two weeks.Conclusion: As expected on the basis of tissue studies, com-parison of proliferation and viability rate of SSCs between healthy and cholestatic rats indicated that the number of stem cells in damaged animals decreases dramatically, and affects their ability to multiply in the medium. The current study shows one of the important side effects of cholestasis on testicular tis-sue and consequent infertility problems in men.

نویسندگان

AH Hasani Fard

Department of Animal Biology, Faculty of Biological Sciences,Kharazmi University, Tehran, Iran

H Mohseni Kouchesfahani

Department of Animal Biology, Faculty of Biological Sciences,Kharazmi University, Tehran, Iran

H Jalali

Department of Animal Biology, Faculty of Biological Sciences,Kharazmi University, Tehran, Iran