Cytotoxic Property of Trifolium Pretens L. Hydroal-coholic Extract On Prostate Cells

Background: Background: Prostate cancer is one of the most common cancers and the second leading cause of cancer-related death among men. It accounts for 33% of all newly diagnosed malignancies, and usually develops over 50 years of age. Tri-folium pratense L. has been suggested for cancer treatment in traditional medicine. In this study the effect of T. pratense hy-droalcoholic extract on two prostate cell lines and normal fibro-blast cells was investigated.Materials and Methods: LNcap and PC3 cell lines were seeded in cell culture flasks containing Roswell Park Memorial Institute 1640 (RPMI-1640) medium supplemented with 10% fetal bovine serum (FBS) without antibiotics. The medium of normal fibroblast cells was DMEM/F12. The cells were treated with 12.5, 25, 50, 100, 200, 400 and 800 μg/mL of T. pratense extract for 24, 48, 72 and 96 hours. Cell viability was evaluated using trypan blue staining, MTT assay, and lactate dehydroge-nase activity measurement. Nitric oxide production was meas-ured using Griess reaction.Results: All the cell lines responded to the cytotoxic effect of the plant extract in a dose- and time-dependent manner. The LNCap cancer cells, however, were more sensitive to the ex-tract as shown by its IC50 values for 24, 48, and 72 hours. Moreover, the extract exhibited selective cytotoxicity in normal fibroblast cells with higher IC50 values. The LDH activity in cell culture medium increased with increasing concentration of the extract. So, cytotoxicity of extract is accomplished with plasma membrane damage. Conclusion: T. pratense showed an anti-cancer property in prostate cancer cell lines.

کلیدواژه ها:

Prostate Cancer ، Cell Culture ، Trifolium Pretense L