Resveratrol Effect on Viability and Nitric Oxide Se-cration in Human Breast Cancer Cell Lines

Background: Breast cancer is the most common cancer of wom-en. Numerous therapies have been identified and used for this cancer. Natural compounds have been used as potential cancer chemopreventive agents. Resveratrol is a natural polyphenolic amzing compound. The aim of present study was to investigate the effect of resveratrol (Res) on viability of breast cancer cell lines (MCF7 and MDA-MB-231) and nitric oxide changes.Materials and Methods: MCF7 and MDA-MB-231 Cells were treated with Res (20μM) during 72 h period. Cell viability (MTT assay), and nitric oxide (NO) production (Griess method) were evluated.Results: Viability of MCF7 and MDA-MB-231 cells were decreased significantly (P=0.000) by Res, After 72 hours and it was 84.84 ± 2.1 %. Nitric oxide production by MCF7 and MDA-MB-231 cells exposed upon Res showed a significant re-duction (P=0.000). NO production from MCF7 and MDA-MB-231cells decreased in a time-dependent manner. It was 22.57± 3.2 μM in MCF7 medium (P=0.001), and 18.41 ± 1.4 μM in MDA-MB-231cell medium (P=0.000).Conclusion: Res decreased NO production in a time-dependent manner and its anti-tumor effect appears to be created through angiogenesis inhibition or induction of cell cycle arrest and ap-optosis in cancer cells.

کلیدواژه ها:

Breast cancer ، Resveratrol ، Nitric Oxide ، Viability