The Most Frequent Genotypes of Human Papilloma-virus in Women From Rasht-Iran

سال انتشار: 1398
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 352

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شناسه ملی سند علمی:

RROYAN20_366

تاریخ نمایه سازی: 29 مهر 1398

چکیده مقاله:

Background: Cervical cancer is one the most frequent cancer among sexually active women. Human papillomavirus (HPV) is the most important cause of cervical cancer. More than eighty subtypes have been identified which can be subdivided to high risk and low risk on the basis of tumorigenicity. There are many studies which implicated that the prevalence of HPV subtypes is related to age and ethnics. The aim of the present study was to evaluate the distribution of HPV in women from Rasht-Iran. Materials and Methods: The present cross sectional study in-volved two thousand women who referred to Jam pathobiology and medical genetic laboratory Rasht-Iran from 2016 to 2018. Two hundred seventy-six patients were identified as HPV posi-tive. Following DNA extraction according to the manufacturer instructions, multiplex polymerase chain reaction (PCR) was performed based on specific primers of low and high risk HPV. Finally, under UV light electrophoresis, PCR products were evaluated. Patients were grouped based on age: <20 (n=3), 20-39 (n=225), 40-59 (n=37), 60-79 (n=2).Results: Of total women, 13.8% were positive for HPV sub-types which 6.5% was high risk. The patient’s age ranged from 18 to 61 with average of 32.5±7.3. Two hundred and sixty-sev-en (94%) of collected specimen were cervical Pap smear and 6% were other genital samples. The most frequent genotype among HPV positive patients were HPV6 (27%) and HPV16 (11%). Eighty four (30.5%) of all patients were infected with multiple HPV subtypes. Women aged 20-50 years had the high-est frequency of HPV subtypes (n=256).Conclusion: HPV6 and HPV16 were the most frequent low and high risk HPV. The prevalence of HPV in women aged 20-50 years was the highest. The identification of ethnic and age related HPV subtypes help clinicians to develop therapeutic and preventive plans and vaccination.

نویسندگان

Z Nikpouri

Mehr Fertility Research Center, Guilan University of Medical Sci-ence, Rasht, Iran

M Mehrafza

Mehr Fertility Research Center, Guilan University of Medical Sci-ence, Rasht, Iran

H Hajizadeh

Jam Pathobiology And Medical Genetic Laboratory, Rasht, Iran

A Raoufi

Mehr Fertility Research Center, Guilan University of Medical Sci-ence, Rasht, Iran