Novel Nanosized Chelator Free 99mTc-Negatively Charged PEGylated Dendrimer-G2 Glycoconjugate as Heart Diagnostic Agent

Background and Objective: Due to the vast growth of the patients’ population with the cardiovascular risk and costs of health care, researches focus on providing novel, safe and cost-effective molecular imaging radiopharmaceuticals. In the present experiment, 99mTc-anionic citric acid based-PEG dendrimer-second generation-glucosamine (99mTc-nano-conjugate) was designed for myocardial viability scan. Method of Evaluation: Dendrimer-G2 was synthesized and conjugation of glucose and dendrimer was prepared thereafter. The synthesis were confirmed by FT-IR, 1HNMR, LC-MS, DLS and SLS. Toxicity on normal cells was assessed by XTT and apoptosis/necrosis methods. In-vivo study including SPECT, dynamic planar imaging and biodistribution were done to confirm the potential imaging of the 99mTc-nano-conjugate Result: Spectroscopy’s confirmed the perfect synthesis of nano-conjugate. Based on data yield by the radiochemical purity (RCP), the anionic dendrimer has a very high potential to complex formation with 99mTc and also is very stable in the human serum in different times. SPECT, dynamic planar imaging and biodistribution demonstrated the suitable tumor uptake of nano-conjugate. Conclusion: Generally, suitable features of the dendrimer including biocompatibility, biodegradability, low toxicity, solubility, low cost, stability and chelator-free radiolabeling of dendrimer with 99mTc, show that it is a promising agent to improve myocardial viability scan.

کلیدواژه ها:

Biodegradable ، Biocompatible ، Dendrimer ، Glucosamine ، Chelator free ، Radiolabeling