Antifungal effect study of Aqueous, Alcoholic, Acetone Extract of the Melissa and Valerian officinalis Effect on Candida albicans and Candida glabrata

Background and Objective: Candida albicans and Candida glabrata are from the group of yeast fungi and cause diseases related to the vagina, gastrointestinal tract, bronchitis, and etc. Today, many drugs available for the treatment of disease. But due to the drug resistance, there is a need for newer drugs to deal with this fungal group, which eliminates the pathogens, don t make any problem for other cells and the body s normal flora. Marjoram and valerian are two herbs that have long been used to treat various diseases. The aim of this research was to investigate the effect of aqueous, ethanolic and acetonic extract of Melissa officinalis against Candida albicans (ATTC 1677) and Candida glabrata (CBS 17750). Materials and Methods: Aqueous, ethanolic and acetonic extract of seeds were prepared by maceration method. The MIC was determinate by macro broth dilution assays and MFC test was used to confirm MIC results. The antioxidant activity of the extract was measured by determination he DPPH. The 0.05 of extracts stock (10, 20, 40, 60, 80 and 100μl) were solved in 10ml of methanol and then mixed with 3ml of diluted 90mm DPPH to bring the 1ml volume of extracts stock. We repeated that’s work for times and the absorbance was read at 517nm. Findings: High concentration of seeds extracts had the increase inhibitory effect. The aqueous extract didn’t show the inhibitory effect on Candida albicans and Candida glabrata. The result showed that the seeds extracts had more antioxidant activity and increased the antioxidant activity in high concentration. Results obtained in this study showed that the inhibitory effects of Melissa officinalis on Candida albicans and Candida glabrata relevant to its antioxidant activity. Conclusion: According to the results of this study, likely the ethanolic and acetonic extracts of seeds Melissa officinalis have antioxidant activities and secondary metabolites.

کلیدواژه ها:

Melissa officinalis ، Candida albicans ، Candida glabrata ، antioxidant