Investigation on the presence and amount of pesticides in eating vegetables consumed in Hamadan in 1397

Introduction: Pesticides have made high contributions in the protection of agriculture crops (Nakano et al., 2016). Pesticides are effective and harmful factors to human health and the environment. Therefore, one of the biggest health concerns is pesticide residue in food products. Today wide-spreading application of pesticide has created serious concerns about adverse effects of these substances on the environment and threat of human health (Miele et al., 2015).Today, pesticide residue in food products, that is effective and harmful factors to human health and the environment, is the one of the health concerns. The evaluation of pesticide residue, it becoming more evident that existence of some pesticides is remarkable, and in some of the studied samples, the range of some pesticides were above of limited of tolerance which is dedicated on WHO standard. The vegetables are one of the consumed food in the world. so the presence of a large amount of pesticides in this product can be considered as an important factor in limiting health. Chlorpyrifos ethyl, diazinon, dimethoate, propanil, iprodione, trichlorfon, Chlorpyrifos methyl were of the widely consumed insecticide and applied to control insect pests on vegetable and agriculture products. The purpose of the present study was to determine the presence and amount of pesticides in the eating vegtable consumed in Hamadan in 1397 using gas chromatography with mass detector (GC/MS/MS).Method: In this study, fourty eating vegtable samples on the markets were randomized and studied in terms of the presence and amount of pesticide: Chlorpyrifos ethyl, diazinon, dimethoate, propanil, iprodione, trichlorfon, Chlorpyrifos methyl as consumed Pesticide. Extraction of the toxins from the specimens was performed by the QuEChERS method. To analysis was used the GC/MS/MS and MRM method. Validation was done by WHO protocole (SANCO2013). All tests were randomly done three times and average of tests were achieved and analysis of variance (ANOVA) was conducted using the linear model (G.L.A) using Minitab (version 17) at the 95% confidence level (p< 0.05) and Duncan test was done.Result: The calibration curves were obtained by plotting the peak area against the concentrations of Chlorpyrifos ethyl, diazinon, dimethoate, propanil, iprodione, and trichlorfon and Chlorpyrifos methyl of the corresponding calibration standard solutions. Linearity regressions (R2) respectively equal to 0.9998, 0.9975, 0.9956, 0.9993, 0.9991, 0.9989, 0.9996 and limit of quantitation (LOQ) respectively equal to 8.3, 7.5, 11.3, 2.7, 5.9, 14.5, 6.8 ppb. The values for Chlorpyrifos ethyl in the range of 10 to 210, for Chlorpyrifos ethyl in the range of 10 to 210, for diazinon in the range of 5 to 270, for dimethoate in the range of 20 to 130, for propanil in the range of 6 to 140, for iprodione in the range of 20 to 80, for trichlorfon in the range of 40 to 70, for Chlorpyrifos methyl in the range of 10 to 380.The LOQ is defined as the lowest fortification level at which acceptable recovery data are obtained. The limit of detection (LOD) of the method is defined as the lowest calibration standard chromatographed having a peak area equivalent to, or better than three times the baseline noise.Conclusion: In previous study he LOD was found to be 0.1 ng (Meekeret et al., 2006). The results showed that 73% of the samples at least were contaminated with one of these toxins. Also, in 7% of samples the values were higher than the WHO set limits. In same research, the highest concentration of chlorpyrifos ethyl 4.23 μg/L was found in the samples and the lower dose resulted in an almost 50% lower concentration of chlorpyrifos ethyl 2.32 μg/L was found (Aprea et al., 2002).