Effect of Intraperitoneal Administration of Silymarin on Cervical Linear Esophagotomy Wound Healing in Rats

Wound healing consists of a complex variety of interactions which finally aid in restoration of tissue function and its elasticity. Wound healing in the esophagus is of great importance due to its specific structure and its positioning adjacent to vital structures, making it the interest of many scientists. Various parts of the plant Silybum. Marianum has been widely used in the folk medicine of Iran and other parts of the world. The active compounds of the plant are constituents of the seeds which include four Flavunolignans collectively known as Silymarin. The aim of the present study was to evaluate the effects of Silymarin on the healing process of experimental wound models of Rats made via esophagotomy incision. Following the esophagotomy, the animals were divided into 3 groups of 7 for post-operational care: Group 1 (control): Received only water during the first 24 hours and then were fed on pelleted diet for 4 days and were finally fed on standard diet until the end of day 14 and remained untreated. Group 2 (solvent): Were kept on the same dietary condition as the control group except that the animals in this group received 5 daily injections of the solvent, DMSO + distilled water (1ml) for five consecutive days. Group 3 (treatment): Received the same diet as the previous group, however were given the same amount of Silymarin + distilled water instead of the sole solvent. Evaluation of the wound healing process was performed clinically, by necropsy and histopathologically 14 days after the onset of the experiment (esophagotomy). Histopathological sections were prepared and the specimens were stained with H&E and Masson Trichrome staining. The results showed that the number of fibroblasts, capillary buds and the organized collagen content of the wound were significantly higher in the treatment group compared to the control and solvent groups (p<0.001). Sub-mucosal tissue evaluation showed inflammation and edema in the control group. The inflammatory cell count was decreased among the groups with a rank order of control > solvent > treatment (p<0.001). Moreover, a significant difference was observed in collagen formation and its consistency between the control and the treatment group (p<0.001). According to the positive effects of intraperitoneal administration of silymarin in the process of cervical linear esophagotomy wound healing in rats, it can be concluded that the use of silymarin can improve and enhance the processes of esophageal wound healing within the indicating period in the rat.

کلیدواژه ها:

Silymarin ، Linear Esophagotomy ، Healing ، Anti-Inflammatory ، Collagen ، Angiogenesis ، Rat