Preparation and Evaluation of Chitosan-Carboxymethyl Cellulose Composite Film with Mentha longifolia Extract for Wound Healing Activity

The skin is the largest organ in the body which protects the internal organs against external injury. When the skin is damaged, microorganisms attack easily and cause infection in humans. Many efforts have been made for development of new products to protect the skin from damage and dehydration. Due to the variety of wound dressing products, it may be wrong to choose wound dressing appropriately. Although, protection of the wound from infection is one of the reasons for using a wound dressing, acceleration of wound healing process is the main goal.[1] Many phytochemical compounds such as phenols, flavonoids, carotenoids, vitamins, tryripsenes, tannins, fatty acids, and amino acids are present in all parts of the plant with biological activity. These natural factors improve wound healing and regenerate the lost proteins in the burn.[2,3] The aim of this study was to investigate the antibacterial and antifungal properties of Mentha longifolia extract on chitosan-carboxymethyl cellulose composite bed for wound making. For this purpose, the effect of the ethanol extract of M.longifolia on the composite film bed of chitosan-carboxymethyl cellulose was investigated. Concentrations of 5%, 10%, 20% ethanolic extracts of M. longifolia leaves with control sample (pure film) were investigated. The results of the experiments and the tests showed that M. longifolia extract has antimicrobial properties and is resistant to gram-positive bacteria in the presence of 20% of the extract on the film substrate, more than the sample with concentration Lower extract and pure chitosan-carboxymethyl cellulose film. Also, the presence of M. longifolia extract with 40% concentration increased the water absorption rate and swelling rate. Surface morphology also showed that increasing the concentration of extract in the film can improve the healing activity of the wound.

کلیدواژه ها:

Wound Dressing ، Biopolymer ، Composite ، Chitosan-Carboxymethyl Cellulose ، Mentha longifolia Extract