Comparison of Islamic Traditional Prescriptions (Iranian vs. Iraqi) for Diabetes Treatment Hitosan / Modified Egg Shell Wound Healing Scaffold Containing Vitamin D and Herbal Remedy for Diabetic Wounds

سال انتشار: 1397
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 404

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شناسه ملی سند علمی:

WTRMED05_067

تاریخ نمایه سازی: 5 آذر 1397

چکیده مقاله:

Aim and Background: Diabetes mellitus is categorized as a metabolic disease, caused by disorders of the level of glucose in the blood Research has shown that alpha –amylase and alpha-glucosidase enzymes play an important role in regulating the amount of glucose in the blood that they can lower blood glucose levels and control the disease if they are inhibited. A group of plants with their own chemicals can target the active site of enzymes and reduce the enzyme activity by reducing activity or inhibition. This article focuses on the use of traditional Iranian and Iraqi medicinal prescription in the use of indigenous plants to control that alpha–amylase and alpha-glucosidase enzymes to control diabetes. Methods: Initially, the plant samples were dried and extracted. The extraction process was done in such a way that the samples were crushed, milled, mixed together and dissolved using different solvents. Finally, the extracts were prepared using a rotary and freeze-dried powder. Upon completion of the extraction, alpha–amylase and alpha-glucosidase enzymes affect them and measure their inhibition that this is achieved by producing a color effect and absorbing enzymes at certain wavelengths. The extract having the ability to inhibit the enzyme more than 70% monitor for their inhibitory separated compound using high performance thin layer chromatography (HPTLC) on-plate enzyme assay. The resulted compound removed from the plate and Identified using LC-ESI/MS. Results and Discussion: The results obtained from the enzymatic tests of various extracts as well as HPTLC on-plate, indicate that the extract containing Prunus Dulcis and Prunus Mahaleb extract with an inhibitory effect of about 75 to 80% can inhibit both the enzyme and reduce its activity. In the case of the HPTLC spectrum, it was also found that the extracts examined at 366 nm wavelengths had certain bands in different parts of the plate, and when we put these plates in the enzymatic action, we found that the enzyme inhibitory function is evident in the extracts loaded in the plates. The identified compounds belong to hydrophilic parts of the prescription. Conclusion: The results of this work showed that extract from indigenous plants in Iran and Iraq has the potential to inhibit important enzymes in the control of diabetes, and they can be used as drugs for the treatment of diabetes.

کلیدواژه ها:

Diabetes ، Traditional Medicine ، alpha-glycosidase ، alpha-amylase ، On-plate HPTLC Enzyme Assay

نویسندگان

Kareem Hashim Albaidhani Rasha

Msc Student of Biochemistry Protein Research Center (PRC), Shahid Beheshti University, G.C., Tehran, Iran

Fateme Mirzajani

Msc Student of Biochemistry Protein Research Center (PRC), Shahid Beheshti University, G.C., Tehran, Iran

Amin Hamidi

Msc Student of Biochemistry Protein Research Center (PRC), Shahid Beheshti University, G.C., Tehran, Iran

Hassan Rezadoost

Msc Student of Biochemistry Protein Research Center (PRC), Shahid Beheshti University, G.C., Tehran, Iran