Comparative Evaluation of ARTADERM 1 and Cod Liver Oil Ointment on Healing Process in Burn of the Second Degree in Rat

Background: The treatment of skin burn is one of the most important challenges in medical science. Burns are divided into three degrees and each one has different effects on skin and other tissues. Many efforts have been applied by clinicians to manage wound healing through using genes, cytokines, chemokines and surgery. Some mechanisms that are involved in the process are local: tissue hypo perfusion, edema, prolonged inflammation, hypercoagulability, free radical damage and accumulation of cytotoxic cytokines. Material and Methods: In this study twenty-eight female Wistar rats weighing 200-250 grams obtained and all of them were kept in controlled temperature of 23-25 oC and light period of 12- hours lightening and 12-hours darkness. Due to make burns after rats’ general anesthesia Ketamine (40 mg/kg) and Xylazine (5 mg/kg) by Intra Protareal (IP) method was performed, after shaving of rats, A brass metal piece 1.4 cm × 3 mm thickness with 100 oC was kept on back of rats for fifteen seconds and it was made similar deep second-degree burns. No significant difference was found among four groups regarding the primary wound surface area. The rats randomly were separated into four groups consisting of seven rats. In the control group the burn injury only was covered with sterile gauze, Artaderm ointment group, cod liver oil group, Eucerin group. The injury area was covered with sterile gauze bandage and this process continued for 21 days. On the 0, 7th, 14 th and 21st day of the experiment, tissue samples were taken under anesthesia from predetermined areas from all subjects in all groups. Wounds were daily examined for any changes in appearance of wounds, the color and smell of any discharge and time of scar separation. Results: Statistical comparison of the burn area of study groups on days 7-14 and 21 post- burn, showed that between the control group and other groups, there was significant difference (P< 0.05). While the best results for the treatment group Artaderm. According to studies, pathology, quickly restored with remarkable Artaderm was a significant difference between the effects of the other drugs. After stained with H & E , the treated group by Artaderm herbal ointment treatment significantly reduced the number of lymphocytes and enhanced the number of fibroblasts at the earlier stages and increased number of fibrocytes at the later stages of wound healing and 1 A herbal ointment improved re-epithelization, fibroblasts proliferation, and collagen bundle synthesis compared to cod liver oil and Eucerin and control group. Conclusion: In the end, according to the results medication Artaderm in terms of healing the wounds and the healing has successfully demonstrated its therapeutic effect, has strong healing properties that can be used as a drug burn along with other existing burn ointments.

کلیدواژه ها:

Second Degree Burns ، Cod liver Oil ، Artaderm Ointment ، Alcoholic Extracts ، Rats