Fabrication of Carboxymethyl chitosan/diopside biocomposite- ZnFe2O3 using freeze drying technique for wound dressing application

Introduction: In this world, a large number of people suffer from skin injuries due to various events such as burns, accidents. Also, the increase in number of people with underlying diseases such as diabetes, causing severe mental and economic problems in the society. Therefore, it is important to reduce economic costs and shorten treatment time, providing strategies that can control the progression of the wound and be effective in healing. Materials and methods: In this study, we aim to improve the mechanical and biological properties of wound dressing using for wound healing approaches. Also, the effect of different weight percentages of ZnFe2O3 magnetic nanoparticles (0, 2/5, 5, and 7 wt%) reinforcement on properties such as strength, bioactivity, biodegradability and antibacterial behavior of Carboxymethyl Chitosan-diopside bio-nanocomposite wound dress was synthesized by freeze drying (FD) method was evaluated. For this purpose, various methods including Fourier Transform Infrared Analysis (FTIR), X-ray diffraction (XRD) and scanning electron microscope (SEM) analysis were used to prove the existence of scaffold elements. In order to determine the antibacterial activity of the fabricated scaffold, Staphylococcus aureus and Escherichia coli were used as test microorganisms. Results: The results showed that using SEM images the wounds dress were porous and had porous interconnections. In fact, according to the results, as the magnetic material increases, the porosity also increases, so that from the first sample (sample without magnetic nanoparticles) to the sample containing the highest amount of magnetic material (7.5 wt%) the porosity increases by 10%. On the other hand, the tensile strength for the pure sample was 0.32 MPa and in the sample containing the highest percentage of magnetic nanoparticles was 0.85 MPa. In addition, the bioactivity and biodegradability of the wounds respectively were immersed in the simulated body solution (SBF) and phosphate buffer solution (PBS). Toxicity by the MTT assay was determined. Also, the antibacterial effect of the wounds on two-gram positive Staphylococcus aureus and Escherichia coli was investigated. The results indicated biocompatibility, good degradation, non-toxicity and acceptable antibacterial properties for the synthesized wound dressing was seen. Also, obtained results show the antibacterial inhibition zone of 12 and 20 mm for Escherichia coli and Staphylococcus aureus, respectively. Discussion and conclusion: According to the results, the selected specimen for mechanical and biological properties is a Carboxymethyl Chitosan-diopside biocompatible wound containing by 5 wt% of Zn-hematite magnetic nanoparticles that is introduced as a suitable candidate for use in wound healing.

کلیدواژه ها:

Bio-nanocomposite ، Carboxymethyl Chitosan ، Diopside ، ZnFe2O3 ، Freeze drying technique ، Tissue engineering ، Wound dressing