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گواهی نمایه سازی مقاله The conformational changes of DNA by mobile phone radiofrequency (940 MHz)

عنوان مقاله: The conformational changes of DNA by mobile phone radiofrequency (940 MHz)
شناسه (COI) مقاله: ICECS01_063
منتشر شده در اولین همایش بین المللی بحران های زیست محیطی ایران و راهکارهای بهبود آن در سال ۱۳۹۱
مشخصات نویسندگان مقاله:

Azadeh Hekmat - Institute of Biochemistry and Biophysics, University of Tehran, Tehran, Iran
Ali Akbar Saboury - Center of Excellence in Biothermodynamics, University of Tehran, Tehran,

خلاصه مقاله:
Currently, the biological effects of nonionizing electromagnetic fields (EMFs) including radiofrequency (RF) radiation have been the subject of numerous experimental and theoretical studies. The aim of this study is to evaluate the possible biological effects of mobile phone RF (940 MHz, 15 V/m and SAR=40 mW/kg) on the structure of DNA immediately after exposure and 2 h after 45 min exposure via diverse range of spectroscopic instruments. The UV-Visible and CD experiments depict that mobile phone EMFs can remarkably cause disturbance on DNA structure. In addition, the DNA samples, immediately after exposure and 2 h after 45 min exposure, are relatively thermally unstable compared to the DNA solution, which was placed in a small shielded box (unexposed DNA). Furthermore, the exposed DNA samples (the DNA samples that were exposed to 940 MHz EMF) have more fluorescence emission when compared with the unexposed DNA, which may have occurred attributable to expansion of the exposed DNA structure. The results of dynamic light scattering (DLS) and zeta potential experiments demonstrate that RF-EMFs lead to increment in the surface charge and size of DNA. The structure of DNA immediately after exposure is not significantly different from the DNA sample 2 h after 45 min exposure. In other words, the EMF-induced conformational changes are irreversible. Collectively, our results reveal that 940 MHz can alter the structure of DNA. The displacement of electrons in DNA by EMFs may lead to conformational changes of DNA and DNA disaggregation. Results from this study could have an important implication on the health effects of RF-EMFs exposure.

کلمات کلیدی:
Mobile phone radiofrequency, Calf thymus DNA, Spectroscopy, Dynamic light scattering (DLS), Zeta potential, Nonionizing electromagnetic fields (EMFs)

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