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گواهی نمایه سازی مقاله Detection of polymorphism of Insulin-like growth factor-I (IGF-I) gene in native Aseel chicken breed of Pakistan using PCR-RFLP

عنوان مقاله: Detection of polymorphism of Insulin-like growth factor-I (IGF-I) gene in native Aseel chicken breed of Pakistan using PCR-RFLP
شناسه (COI) مقاله: JR_AAJ-2-6_002
منتشر شده در ماهنامه پیشرفت های کشاورزی در سال ۱۳۹۲
مشخصات نویسندگان مقاله:

asad ali - Department of Livestock Production, University of veterinary and Animal Sciences, Lahore, Pakistan.
khalid javed - Department of Livestock Production, University of veterinary and Animal Sciences, Lahore, Pakistan.
afzal ali - Department of Livestock Production, University of veterinary and Animal Sciences, Lahore, Pakistan.
Muhammad Akram - Department of poultry Production, University of veterinary and Animal Sciences, Lahore, Pakistan.

خلاصه مقاله:
Molecular analysis is an easier means to identify desirable genotypes for growth. Candidate gene (s) for growth trait like insulin like growth factor (IGF) has imperative function for growth, body composition, fat deposition, metabolic and skeletal traits and the molecular genetic selection on individual genes is a very efficient method to genetically improve economically important traits in chickens. In the present study, polymorphism of the promoter and 5' untranslated region of IGF-I gene of nativeAseel, was investigated. In order to evaluate the IGF-I gene polymorphism, we used the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method. Feather samples were collected at random from 50 Aseel chickensof Pakistan. Genomic DNA was extracted using modified salting-out PCI (Phenol: Chlorophorm: Iso-amylacohol) method and amplified by polymerase chain reaction. The promoter and 5' untranslated region of the IGF-I gene was amplified to produce a 621 bp fragment. The PCR products were electrophoresed on 2.5% agarose gel and stained by ethidium bromide and then finally on confirmation of amplification the ampliconswere digested Hinf-I restriction enzyme and this revealed two alleles A and B having all three combinations of genotypes i.e. AA, AB were found. Data were analyzed using Pop Gene 3.1 software package. Allele frequencies (A and B) were 0.53 and 0.47 while genotypic frequency fo AB (66) was significantly higher than AA (20) and AB (66). The Chi- square (χ2) test did not show any deviation from Hardy–Weinberg equilibrium.

کلمات کلیدی:
Insulin-like growth factor-I (IGF-I) gene Polymerase chain reaction Restriction fragment length polymorphism (RFLP) Aseel Polymorphism

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